Coupling of Endonuclease and Translocase Functions in EcoR124I

Page s 135glycosyl hydrolase with exo-1,3-β-glucanase activity was isolated and its gene subsequently over-expressed in E.coli.The sequence showed it to be a GH3 family member, closely related to barley exo-1,3-1,4-β-glucanase [1] in two of its three domains.This was confirmed by crystallographic analysis which also showed how the additional C-terminal domain interacts with the N-terminal domain.Activity studies demonstrated the extra domain was essential for enzyme activity.