Incident Cases Characterization and Deep Sequencing Provide New Insight into Multiplicity of Infection and HIV Evolution in Very Early Acute Infection

Background RV217/ECHO aims at capturing HIV-1 incident cases during the very earliest stages of acute HIV-1 infection (AHI). Limitations in single genome amplification(SGA) sampling depth have the potential to confound the study of multiplicity of infection and viral evolution during AHI. Here we combined SGA and deep, next-generation sequencing(NGS) to investigate the prevalence of minor viral variants during pre-peak viremia and characterize subsequent viral evolution.