Leishmania infantum trypanothione reductase is a promiscuous enzyme carrying an NADPH:O 2 oxidoreductase activity shared by glutathione reductase

•In the absence of trypanothione, TR displays an NADPH:O2 oxido-reductase activity.•The product of this reaction is water and not hydrogen peroxide.•TR is a promiscuous enzyme which prevents formation of toxic O2 species.•The NADPH:O2 oxido-reductase activity is shared by glutathione reductase.