Facile And Cost-Effective Detection Of Saxitoxin Exploiting Aptamer Structural Switching
FOOD TECHNOLOGY AND BIOTECHNOLOGY(2015)
Abstract
A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 mu g/mL of STX, with a detection limit of 7.5 ng/mL.
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Key words
red tide,paralytic shellfish poison,aptamers,saxitoxin,high resolution melting
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