Simultaneous determination of intracellular UDP-sugars in hyaluronic acid-producing Streptococcus zooepidemicus

Lukas Franke,Dagmar Cožikova,Dzianis Smirnou,Martina Hermannova,Tereza Hanova, Andrea Růžickova,Vladimir Velebný

Journal of Chromatography B（2015）

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摘要

•Determinationof UDP-sugars involved in hyaluronan pathway was accomplished.•Validation results demonstrated high sensitivity and reliability of HPLC methods.•Methods were used to monitor UDP-sugar concentrations in SEZ cells extracts.•Intracellular UDP-sugars pools varied in different SEZ growth phases.

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关键词

Hyaluronan,UDP-sugars,Ion exchange chromatography

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