Extended proteome and membrane subproteome of mycoplasma genitalium

Mycoplasmas represent one of the simplest biological systems and are good examples of a true autoreplicative minimum cell. The proteome of Mycoplasma genitalium has been analyzed in deep using gel-based and gel-free technologies. The proteome has been analyzed in the exponential growth phase and we have achieved the identification of 343 proteins (70.4%) of the 487 predicted ORF, being 274 of these proteins not identified in previous proteomic analysis.