Endothelial Nitric Oxide Synthase Gene Polymorphism (T-786 C) in Sudanese Patients with Sickle Cell Anaemia

Background and Objective: Endothelial nitric oxide synthase gene polymorphisms have been associated with the sickle cell anemia. The aim of conducting this study was to explore the association between the single nucleotide polymorphism (T-786C) of endothelial nitric oxide synthase gene and Sickle cell anaemia among Sudanese patients. Method: This was a case control study in which a total of 127 patients with sickle cell disease and 88 Healthy controls were involved. The single nucleotide polymorphism (T-786C) was genotyped by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism and the association was tested by Haploview software. Result: The distribution of mutant allele -786C was present in 5.5% of patients and completely absent in control subjects, but there is no significant association between single nucleotide polymorphism (T-786C) and Sickle cell Disease in Sudan (p>0.05). Conclusions: This study indicated lack of association between single nucleotide polymorphism (T-786C) and Sickle cell Disease among Sudanese patients.