Isolation and identification of pathogenic fungi causing decay in Luotian chestnut during late storage period and research on pathogenicity.

In order to explore moldy mechanism of chestnut from Luotian County in storage process,the strains of pathogenic fungi were isolated from chestnuts after storage at room temperature for 70d.Six genera of fungi were found in chestnut through experimental identification,which were Ozoniumsp.,Fusarium sp.,Aspergillus sp.,Penicilliumsp.,Rhiopus sp.and Stachybotrys sp.,respectively.The re-inoculation tests had been conducted on pathogenic fungi whose isolating rate was greater than 10%.The result showed that the rest genera of fungi generally had no pathogenicity except Penicilliumsp.could infect non-injured chestnut with a lower moldy rate and lighter symptoms;but the moldy rate of strains was above 60% in injured inoculation and they showed heavy symptoms,among which the moldy rate of Ozoniumsp.and Aspergillus sp.were higher than 80%.The experimental results showed that injured chestnut were more likely to decay.Ozoniumsp.and Aspergillus sp.were important pathogenic fungi causing decay during storage process of chestnut.