Production of wild Chlorella sp. cultivated in digested and membrane-pretreated swine manure derived from a full-scale operation plant

A wild Chlorella sp. was isolated from a manure storage tank and then cultivated under semi-continuous production conditions on four substrates, including digested swine manure (DIG) and digestate-derived liquid fractions obtained by digestate centrifugation (CLF) and ultrafiltration (ULF) in a full-scale digestate treatment plant, in order to evaluate differences in growth and depuration capacities. The microalga was capable of fast growth on some of the substrates, comparable to that obtained with 3N-BBM synthetic medium, productivities being 0.21gL−1d−1 of biomass for ULF and 3N-BBM media, 0.17gL−1d−1 biomass for CLF media and 0.10gL−1d−1 biomass for DIG media.