Protective Effects of Withania Somnifera Root on Inflammatory Markers and Insulin Resistance in Fructose-Fed Rats

Background: We investigated the effects of Withania somnifera root (WS) on insulin resistance, tumor necrosis factor alpha (TNF-alpha), and interleukin-6 (IL-6) in fructose-fed rats.Methods: Forty-eight Wistar-Albino male rats were randomly divided into four groups (n=12); Group I as control, Group II as sham-treated with WS by 62.5mg/g per diet, Group III fructose-fed rats received 10% W/V fructose, and Group IV fructose- and WS-fed rats. After eight weeks blood samples were collected to measure glucose, insulin, IL-6, and TNF-alpha levels in sera.Results: Blood glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-R), IL-6, and TNF-alpha levels were all significantly greater in the fructose-fed rats than in the controls. Treatment with WS significantly (P < 0.05) inhibited the fructose-induced increases in glucose, insulin, HOMA-R, IL-6, and TNF-alpha.Conclusion: Our data suggest that WS normalizes hyperglycemia in fructose-fed rats by reducing inflammatory markers and improving insulin sensitivity.