Assessment of the Sensitivity and Accuracy of Immunochromatographic Test Strips in the Qualitative Detection of Aflatoxin Contamination in Peanuts

Despite the availability of several mechanical and chemical methods for aflatoxin detection, most are not accessible to many developing countries. In these developing countries, immunochromatographic test strips can be an alternative. Research was conducted to assess the sensitivity and accuracy of immunochromatographic test strips in the qualitative detection of aflatoxin at a 20 ppb cut-off limit as compared to the standard fluorometry method, and to evaluate the effect of continuous high or fluctuating temperatures to the sensitivity and accuracy of the test strips. Results showed significant association (P<0.0001) between the results obtained from the test strips (AflaCheckTM, Vicam) and the Vicam fluorometric method, producing an overall accuracy of 97%, sensitivity of 94%, and specificity of 100%. When exposed to continuous high (34 C) and fluctuating (34 C for 8 hr, 25 C for 16 hr daily) temperatures, AflaCheckTM and AgraStrip® (Romer Labs) retained their ability to detect aflatoxin levels up to 32 and 47 wk (8 and 12 months), respectively, without loss of efficacy. This documents the reliable use of the test strips in tropical peanut production areas where technologies like the fluorometer for aflatoxin quantification and refrigeration for storage are not readily accessible.