The Effect of Red Fruit Oil Toward NF-kB Expression on Colitis-Associated Cancer Mice Model

Introduction: Colorectal cancer has provided a paradigm for the association between inflammation and cancer. Activation of nuclear factor-kB (NF-kB) is associated with various type of cancers due to its key role in innate immunity, inflammation, cell proliferation, and cell survival. Red Fruit (Pandanus conoideus Lam.) which contains large amount of antioxidant has been considered as phytopreventive agent. The aim of this research is to examine the effect of buah merah oil towards NF-kB expression in colitis-associated cancer (CAC) mice model. Methods: BALB/c male mice were divided into four groups (n=6). The negative control and red fruit control groups were given aquabidest and red fruit oil, respectively, without CAC induction. The azoxymethane (AOM)/dextran sulfate sodium (DSS) control and red fruit treatment groups were given AOM followed by DSS to induce CAC. The AOM/DSS control group were given aquabidest while the red fruit treatment group were given buah merah oil. NF-kB colon level was measured using Western blot method. Data were analyzed by One-Way ANOVA continued with Tukey HSD (a=0.05). Result: The result showed that the average NF-kB level of red fruit treatment group was significantly decreased compared to the AOM/DSS control group (p<0.000). Conclusion: Red fruit oil decreased NF-kB level in CAC mice model.J Indon Med Assoc.2013;63:106-11 Keywords: inflammation, colorectal cancer, NF-kB, red fruit