Expression Profile Of Claudin Family Members In The Developing Lung

CF-32The Vermont Cancer Center DNA Analysis Facility provides an array of fast, affordable, user-friendly DNA analysis services to the members of the Vermont Cancer Center and the University of Vermont research community and region. Primary services offered for a fee include DNA sequencing, real-time quantitative PCR, DNA fragment analysis (microsatellite instability, haplotyping, AFLP, and T-RFLP), SNP detection, nucleic acid extraction, image analysis (phosphorimaging, fluorescent detection, DNA and protein stain imaging, chemiluminescence, chemifluorescence) and densitometry), and protein gel spot excision with a BioRad EXQuest Spot Cutter. The facility provides a variety of other important benefits free of charge to our users. These include access to two NanoDrop Spectrophotometers, two MJ Research Gradient Cyclers, and sponsoring seminars on new technologies, holding tutorials on software and user driven instrumentation, primer and probe design, obtaining discounted pricing on common reagents, and providing data analysis, troubleshooting and consultation in experimental design. Our annual Open House is a popular day long event highlighting all of our services. In January 2007, the facility began a new User Educational Seminar Series with the goal to meet with users on a monthly basis to educate and promote services currently being provided in the facility. A computer workstation area is adjacent to the main lab and has freeware and purchased programs available for data analysis and review with full support from nearby facility staff. The facility uses a bioinformatic platform known as the UVM BioDesktop to facilitate the workflow for both the users and the staff of the facility. Users of the facility create a password protected BioDesktop account for online ordering of all services, viewing and archiving data, and accessing data analysis tools. The facility staff uses their BioDesktop account for uploading completed data, creating sample sheets for the instruments, exporting billing information, and tracking completion of orders.