Trichothecene-mediated in vitro selection in wheat for reduced mycotoxin accumulation caused by Fusarium graminearum
CANADIAN JOURNAL OF PLANT SCIENCE(2008)
Abstract
Eudes, F., Comeau, A., Rioux, S. and Collin, J. 2008. Trichothecene-mediated in vitro selection in wheat for reduced mycotoxin accumulation caused by Fusarium graminearum. Can. J. Plant Sci. 88: 1115-1125. Trichothecene, a factor of aggressiveness of Fusarium graminearum in wheat fusarium head blight (FHB), was evaluated in an anther co-culture assay for the regeneration of doubled haploid (DH) lines with reduced mycotoxin accumulation. A Fusarium graminearum culture filtrate and a defined mixture of purified trichothecenes were compared with a control treatment in two F(1)-derived microspores populations. Frontana and Katepwa were the FHB resistant and intermediate resistant sources, respectively, and the cultivar Norseman was the FHB susceptible parent. A preliminary evaluation of the subpopulations of DH lines, using the point inoculation method in the greenhouse, showed selection effects for FHB resistance in the trichothecene co-cultured Frontana/Norseman subpopulation only. Three years of field evaluation using the spray inoculation method revealed that the DH subpopulation from the F, hybrid Frontana/Norseman co-culture in the presence of trichothecenes accumulated consistently less deoxynivalenol (DON) in the grain than the control subpopulation. The FHB symptoms were also significantly reduced for 1 yr (2001) in the same subpopulation. This subpopulation showed increased test weight, plant height and a 1.1-d delay in heading date when compared with the control subpopulation, under disease pressure. A trichothecene co-cultured DH subpopulation from Katepwa/Norseman also had a significantly lower DON content for 1 yr.
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Key words
Androgenesis,disease resistance,Gibberella zeae,mycotoxin,Triticum aestivum,wheat scab
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