Immobilization of reactive dyes on several matrices that allow high and very high flow-rates to be used

Several matrices were used for immobilization of the reactive dye Procion Red H3B: TSK HW 40, 65 and 55, Trisacryl GF 2000, Sephacryl S-200, Superose 6, Sepharose CL-4B and laboratory-prepared dextran-coated Spherosil XO15. The amounts of incorporated dye and the flow properties of the various supports were determined, in addition to their capacity for a trypsin inhibitor present in a cornderived food product. It was shown that dextran-coated silica, TSK HW 55 F, Superpose 6 and Sephadex G-50 F allowed immobilized dye chromatographic supports suitable for purification of this protein under high flow-rate conditions to be prepared. Actual results of purification obtained using dyed dextran-coated silica and dyed Sephadex G-50 F (settled in a squat column) are described and shown to compare favourably with those of a more traditional purification procedure.