Viability of human umbilical cord–derived mesenchymal stem cells in G-rich and M-rich alginates

In this study, the effect of pharmaceutical-grade alginates on the cell viability of human mesenchymal stem cells derived from umbilical cord was examined and their use in tissue engineering applications was evaluated. The effects of the ratio of the copolymer building blocks (guluronic and mannuronic acids) and their interactions with divalent calcium, the purity of alginates (proteins and polyphenol content), and gelation factors (calcium concentration and sol content) were examined. The high guluronic acid content in the alginates improved the viability of the human mesenchymal stem cells derived from umbilical cord and supported cell growth significantly. It was confirmed that the sol fraction of alginate reduced cell viability. Cells in the presence of alginate beads cross-linked with 50 and 100 mM calcium chloride showed maximum viability; the protein and polyphenol content of the alginates did not affect the viability of the human mesenchymal stem cells derived from umbilical cord, while the monomer ratio did have an obvious effect.