[Simultaneous Determination of Index Components in Wuwei Shaji Oral Liquid by HPLC].

OBJECTIVE:To establish an HPLC method of detecting geniposide, mono-ammonium glycyrrhizinate, liquiritin, costunolide and dehydrocostus lactone in the Wuwei Shaji oral liquid simutaneously.METHODS:Diamonsil C18 (2) column(250 mm x 4. 6 mm, 5 µm) was used to analyze above components with mobile phase of acetonitrile(A)-0. 2 phosphoric acid(B) in gradient elution mode at the flow rate of 1. 0 mL/min under wavelength of 220 nm.RESULTS:There was a good linear relationship of the five components, the RSD of the accuracy, repeatability, stability were all bellow 3% and the average recovery were 99. 95%, 95. 00%,100. 26%, 99. 82%, 101. 33%.CONCLUSION:This method is convenience, accuracy and specific,which can be a further supplement for the quality control of Wuwei Shaji oral liquid.