Dokdonia Pacifica Sp Nov., Isolated From Seawater

A Gram-stain-negative, aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, rod-shaped, yellow-pigmented bacterium, designated strain SW230(T), was isolated from a surface seawater sample collected from the South Pacific Gyre. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SW230(T) shared highest similarity with members of the genus Dokdonia (95.0-94.5%), exhibiting 95.0% sequence similarity to Dokdonia genika NBRC 100811(T). Optimal growth occurred in the presence of 2-3% (w/v) NaCl, at pH 8.0 and at 28 degrees C. The DNA G+C content of strain SW230T was 36 mol%. The major fatty acids (>10% of the total) were iso-C-15:1 G, iso-C-15:0, iso-C-17:0 3-OH, and C-16:1 omega 7c and/or C-16:1 omega 6c. The major respiratory quinone was menaquinone-6. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. On the basis of data from the present polyphasic study, strain SW230(T) is considered to represent a novel species of the genus Dokdonia, for which the name Dokdonia pacifica sp. nov. is proposed. The type strain is SW230(T) (=CGMCC 1.12184(T)=JCM 18216(T)).