Effect of plant growth regulators, explant type on plantlet regeneration through callus induction in Nerium oleander L.

It was to cultured leaf explant separated from the mature oleander's plant on MS media. Equipped with 2,4-D at 2mg/l concentration and Kin at 1mg/l concentration. which give the highest percentage of callus induction and fresh and dry weight, but this callus had failed to differentiate into branches when cultured in regeneration media equipped with BA and IAA in different concentration. When cultured stem separated from oleander seedlings. Had given the highest percentage rate of callus induction and fresh and dry weight, when cultured in MS media equipped with Kin and NAA at 1.5mg/l concentration for each. And resulted carryout in the cultured of this callus in regeneration medium equipped with BA and NAA at 2 and 0.5mg/l concentration respectively, to differentiation to the highest rate of the number of branches with this combination. Cultured of this branches in MS media was equipped with IAA at 2mg/l concentration had given the highest percentage of rooting, the number and length of the roots. Highest percentage of success acclimatization when cultured these plantlet in media, consisting one size of sand to one size of Peat moos.