[Establishment and Clinical Application of TapMan Real-Time RT-PCR Method for Detection of HHV-6].

OBJECTIVE:To establish a rapid, sensitive and specific real-time PCR method for detection of Human Herpesvirus-6 (HHV-6).METHODS:According to the reference, a pair of primers and a probe were designed located in U65-66 gene and to set up the standards. We established a real-time RT-PCR method for detection of HHV-6, and to verify the specificity, sensitivity, reproducibility.RESULTS:The correlation coefficient was 0.999, E = 97.9%, the coefficient of variation values of Ct were 0.61% and 3.13% in real-time PCR assay for inter and intra assay, respectively. The results of all viruses were negative except of HHV-6 for the assay. The quantitative detection limit of the assay was 3 x 10(0) copies/microl.CONCLUSION:The real-time PCR assay is highly specific, sensitive and reproducible, which can be used to quatitative detecting clinical samples.