An FTIR-DRIFT study on river sediment particle structure: implications for biofilm dynamics and pollutant binding.

Diffuse reflectance infrared Fourier transform (DRIFT) spectrometry was applied to a set of sediment samples collected by traps over one and a half years in a mid-mountainous river. Dynamic changes in hydrological and life-cycle conditions generated sediment particles of different C-org content and organic composition. Periods in the midst of or shortly after flood events left particles poor in Corg content with spectral features that were enriched in carboxylic and aromatic signals. These are characteristic of terrestrial oxidized vascular plant debris. Low-flow conditions saw the consequent build-up of amide, aliphatic, and polysaccharide moieties as expected for autochthonous biofilm derived material. A peak ratio of two bands representing the alternation of these two types of organic matter showed that flood particle C-org had a higher affinity for metals than the high C-org of mature biofilms, probably owing to higher C00(-) contents in the first. The relative dietary bioavailability of the metals from sediment C-org, which is related to the nutritional value of the substrate, is therefore probably lower in the aftermath of a flood than in prolonged low-flow situations. This needs to be accounted for in future metal speciation and bioavailability modeling approaches.