Characterization of ribosomal binding and antibacterial activities using two orthogonal high-throughput screens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY(2013)
摘要
We report here the affinity and antibacterial activity of a structurally similar class of neomycin dimers. The affinity of the dimer library for rRNA was established by using a screen that measures the displacement of fluorescein-neomycin (F-neo) probe from RNA. A rapid growth inhibition assay using a single drug concentration was used to examine the antibacterial activity. The structure-activity relationship data were then rapidly analyzed using a two-dimensional ribosomal binding-bacterial inhibition plot analysis.
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关键词
Microbicidal Activity,RNA Structure
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