Effect of water quality in mosquito breeding sites on the pathogenicity and infectivity of zoospores from the fungus Leptolegnia chapmanii (Straminipila: Peronosporomycetes)]

Leptolegnia chapmanii (Straminipila: Peronospormycetes). The fungus Leptolegnia chapmanii is highly pathogenic to mosquito larvae in Argentina. We studied if physical and chemical characteristics of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L. chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools filled by overflow from Rio de la Plata, and flower vases From the Cemetery of La Plata city. Sub-samples of water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory bioassays. Containers with 150 in of water samples, 25 Aedes aegypti larvae, and 2.8 x 10(5) zoospores of L. chapmanii, were incubated under controlled environment, and larval mortality was recorded after 48 h. There were highly significant differences among mortalities in water from cemetery, vases (70.2%), rain pools water (99.5%), and pools with water from Rio de la Plata (95%). There were no significant differences among larval mortalities in water from ditches, rain pools and Rio de la Plata pools. Leptolegnia chapmanii was successful as a biological control agent in all kinds of tested water qualities, producing high larval mortality, Rev. Biol. Trop, 57 (1-2): 371-380. Epub 2009 June 30.