Expression of the Human Papillomavirus Type 58L1 Capsid Protein in E.coli and Preparation of the HPV58 L1 Mouse Antiserum

BACKGROUND:Human papillomavirus type 58 (HPV58) has been shown to be one of the most important highly-oncogenic, risky HPV types. The present study aimed at investigating the expression of the HPV58 L1 major capsid protein by E.coli and preparation of HPV58 L1 antiserum.METHODS:The full length L1 coden region of HPV58 L1 was amplified by PCR, the cloned, sequenced. The expression vector pRSETB58 L1 was constructed to produce HPV58 L1 protein. The protein expressed was purified by SDS-PAGE, and used to immunize BALB/c mice.RESULTS:HPV58 L1 protein was expressed in E. coli, which has cross reaction with anti-HPV16 L1 antibody. The mouse anti-HPV58 L1 antibody was obtained and used to test the HPV58 L1 protein expressed in insect cell.CONCLUSIONS:HPV58 L1 major capsid protein was efficiently expressed in E. coli. The mouse anti-HPV58 L1 specific antibody was prepared, which can be used to test the HPV58 L1 protein expressed in eukaryotic cell.