Enterococcus Ureilyticus Sp Nov And Enterococcus Rotai Sp Nov., Two Urease-Producing Enterococci From The Environment

A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus Enterococcus with validly published names and placed them in the Enterococcus faecalis species group. DNA-DNA hybridization experiments, pheS and rpoA sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus Enterococcus, for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629(T) =LMG 26676(T) =CCUG 48799(T)), inhabiting water and plants, and Enterococcus Total sp. nov. (type strain CCM 4630(T) =LMG 26678(T) =CCUG 61593(T)), inhabiting water, insects (mosquitoes) and plants, are proposed.