Enhanced yeast one-hybrid assays for high-throughput gene-centered regulatory network mapping

The authors describe the enhanced yeast one-hybrid platform for large-scale screening of protein-DNA interactions and test its performance by mapping Caenorhabditis elegans gene regulatory networks. Also in this issue, Hens et al . describe an alternative platform for this purpose and apply it to screen for transcription factor–DNA interactions in Drosophila melanogaster .