A nonlinear cell electroporation model and its verification using micro electroporation chips
Nano/Micro Engineered and Molecular Systems（2014）
摘要
A nonlinear electromechanical coupled model is proposed to study the pore expansion of single-cell electroporation (EP) due to intense applied electric field. By introducing the strain energy accumulated in the membrane during EP, the proposed model can predict the critical transmembrane potential, the activation energy, and the equilibrium pore size. To verirfy the model, a 3D single-cell micro EP chip was developed by using micro electroplating technology, and the EP experiments were conducted on chicken red blood cells (RBC). An EP “phase diagram” for chicken RBCs was obtained, which delineates the boundary of the areas for cell lysis, electroporation and absence of electroporation. The critical transmembrane potential of EP predicted from our model shows a good agreement with the experimental results.
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关键词
biomems,bioelectric potentials,biomembranes,blood,electrophoresis,electroplating,lab-on-a-chip,3d single-cell micro ep chip,ep phase diagram,activation energy,area boundary,cell lysis,chicken rbc,chicken red blood cells,critical transmembrane potential,equilibrium pore size,intense applied electric field,microelectroplating technology,microelectroporation chips,nonlinear cell electroporation model,nonlinear electromechanical coupled model,pore expansion,single-cell electroporation,strain energy,electromechanical coupled model,electroporation,micro electroporation chip,red blood cell,lab on a chip,electric fields,electric potential,gold,fluorescence,strain,electrodes
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