A PDMS Microfluidic Impedance Immunosensor for E. Coli O157:H7 and Staphylococcus Aureus Detection Via Antibody-Immobilized Nanoporous Membrane

In this article, a PDMS microfluidic immunosensor integrated with specific antibody immobilized alumina nanoporous membrane was developed for rapid detection of foodborne pathogens Escherichia coli O157:H7 and Staphylococcus aureus with electrochemical impedance spectrum. Firstly, antibodies to the targeted bacteria were covalently immobilized on the nanoporous alumina membranes via self assembled (3-glycidoxypropyl)trimethoxysilane (GPMS) silane. Then, the impedance spectrum was recorded for bacteria detection ranging from 1Hz to 100kHz. The maximum impedance amplitude change for these two food pathogens was around 100Hz. This microfluidic immunosensor based on nanoporous membrane impedance spectrum could achieve rapid bacteria detection within 2h with a high sensitivity of 102CFU/ml. Cross-bacteria experiments for E. coli O157:H7 and S. aureus were also explored to testify the specificity. The results showed that impedance amplitude at 100Hz had a significant reduction in binding of bacteria when the membrane was exposed to non-specific bacteria.