Development of a polyclonal antibody based immunoassay for detection of molybdenum levels in environmental water
Proceedings of 2011 3rd International Conference on Awareness Science and Technology, iCAST 2011(2011)
摘要
An indirect competitive ELISA (icELISA) based on anti-mice polyclonal antibody has been developed for the determination of molybdenum (Mo) levels in environmental water. For this reason, isothiocyanate method was employed to synthesize the artificial antigen of Mo-ITCBE-BSA, and Balb/C mice were used to prepare the polyclonal antibody. Based on the square matrix titration, an icELISA method was developed. The dynamic range was from 0.16 to 78 ng/mL, with LOD and IC 50 value of 0.08 ng/mL and 3.2 ng/mL, respectively. Except for cross-reactivity to Co 2+ (36.3%) and Hg 2+ (42.5%), negligible cross-reactivity to other metal ions was observed. Under optimization dilution folds, the recoveries of Mo 2+ were in the range of 95.3-117.5%, 88.7-113.5% and 97.5-104.2% for tap water, river water and lake water, respectively. Therefore, this assay has the potential to be incorporated into a quantitative monitoring program for the rapid screening of molybdenum levels in water. © 2011 IEEE.
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关键词
artificial antigen,environmental water,indirect competitive elisa,molybdenum,polyclonal antibody,proteins,geochemistry,immune system,biotechnology,nitrogen
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