Biodegradable poly(lactic-co-glycolic acid) Maxpol-T/8S as novel scaffold for adipose derived stem cells and fibroblast growth in vitro

To explore the ability of MaxPol-T/S, a novelbiodegradable poly-lactic-co-glycolic acid(PLGA) scaffold, in providing a cell-matrix interaction interface for cell growth, we generated GFP positive adipose derived stem cell(ADSC(GFP+)) and fibroblasts(FCGFP+) for investigating the cellular growth on this biomaterial. The MaxPol-T/S was produced through salt-leaching/particulate-leaching technology for tissue engineering, which provides a modified surface for the best cellular attachment sites and nutrient supply and waste exchange conditions. The morphological features of MaxPol-T/S were studied with a scanning electron microscope and the ADSC(GFP+) and FCGFP+ were confirmed by auto-fluorescence microscopy and flow cytometry assay. Being seeded onto the MaxPol-T/S in vitro, the growth and morphology of ADSC(GFP+) and FCGFP+ were further verified by auto-fluorescence microscopy and MMT test. The ADSCs and fibroblasts, expressed strong GFP signals in the cytoplasm and nucleus, adhered and proliferated on the surface of scaffold MaxPol-T/S. Both cell lines survived on the scaffold more than 21 days in vitro and formed three-dimensional colonies on the surface of the MaxPol-T. In conclusion, MaxPol-T/S is a novel PLGA scaffold for ADSC(GFP+) and FCGFP+ and implies a promising technique for tissue engineering.