IN VITRO ASSESSMENT OF APOPTOSIS IN AVIAN LYMPHOCYTES TREATED WITH FENVELERATE VIS-À-VIS IMMUNOSUPPRESSION IN POULTRY

Fenvelerate, a synthetic pyrethroid insecticide is used against a wide variety of insect pests in various agricultural, animal husbandry and public health operations. Indiscriminate and disproportionate use of pesticides has lead to their residues resulting in immunological disorders on man and animal. These days, a major concern of most of the researchers is to find alternative methods of animal experimentation. In vitro cellular models can be useful for immunopathological research and have predictive potential in the assessment of toxicity and consequently, safety evaluation. The main advantage of using in vitro cell culture system is that experimental conditions can be strictly controlled without causing any kind of pain to animals and ease for analysis of pathogenic mechanisms of action at cellular level. With the aim of replacing animal models with that of cell culture system in immunopathological studies, it was proposed to carry out the pesticide induced immunopathological studies in lymphocyte cell cultures.