In vitro regeneration of adult trees of Bambusa vulgaris

We developed procedures for the regeneration of Bambusa vulgaris using nodal segments from adult plants. Optimal shoot growth was after 16 days cultivation on modified Murashige and Skoog (MMS) medium supplemented with 2 mg/l of BAP. Elongated shoots of B. vulgaris rooted (45.85%) when cultured in MMS + 20 mg/l IBA. The rooted and acclimated shoots were successfully transferred into the field with 100% of plantlets survival.