Fermentation of Spartina anglica acid hydrolysate by Trichosporon cutaneum for microbial lipid production

The bioconversion of partially detoxified acid hydrolysate of Spartina anglica to lipid by Trichosporon cutaneum was studied.The hydrolysis of S.anglica at 122 ℃,using four concentrations of sulfuric acid(1%,2%,4% and 6%),was investigated with a solid-liquid ratio of 10%.Maximal total reducing sugar was realized with 6% sulfuric acid for 40 min.Moreover,at 140 ℃ using 1% sulfuric acid,glucose was 14.2 g/L.The optimum concentrating factor of hydrolysate was 4.0.Under these conditions,the lipid was produced up to 5.9 g/L.This work provided valuable information for development of an efficient process to produce microbial lipid from Spartina anglica acid hydrolysate.