Anca Auto-Antibodies In Children With Cystic Fibrosis Are Not Associated With Genetic Polymorphisms Of Tgf Beta1 And Tnf Alpha Cytokines

To investigate the contribution of the −238G/A and −308G/A tumor necrosis factor (TNF) α, and +252A/G lymphotoxin (LT) α gene polymorphisms to idiopathic recurrent miscarriage (RM).A retrospective case-control study.Outpatient maternity center.Study subjects comprised 372 RM women and 274 age-matched parous control women.None.The TNFα and LTα gene variants and idiopathic RM.Higher prevalence of TNFα −238A and LTα +252G alleles and LTα +252G/G genotype and lower frequencies of TNFα −308G/A were seen in RM cases. Three-loci haplotype analysis (TNFα −308GA/TNFα −238GA/LTα +252AG) demonstrated significant association between TNFα-LTα gene variants and RM. Both protective [−308A/−238G/+252A], and susceptible [−308G/−238A/+252G] haplotypes were identified. Mutlivariate regression analysis confirmed the association of −308G/−238A/+252G haplotype with exclusively early RM, after controlling for a number of covariates; no specific TNFα and LTα genotypes or haplotypes were linked with either late or combined early and late RM.The TNFα −238G/A and LTα +252A/G, but not TNFα −308G/A, polymorphic variants are associated with exclusively early idiopathic RM.