Identification and Quantification of Trans-Resveratrol in Dietary Supplements by a Rapid and Straightforward RP-HPLC Method

This paper reports the results of a study performed to develop a rapid and straightforward chromatographic method for the identification and quantification of trans-resveratrol in commercial trans-resveratrol containing dietary supplements. The method employs an Alltech Platinum EPS 100 A C-18 column operated under isocratic elution mode with a 70:30 (v/v) water-acetonitrile mixture as the mobile phase, at flow rate of 1.0mL min(-1). Peak detection is performed at 306nm with limit of detection (LOD) of 0.27 mu gmL(-1) and limit of quantification (LOQ) of 0.95 mu gmL(-1) and linear calibration graph within the concentration range of 2.63 to 10.53 mu gmL(-1). Other data related to the successful method validation include representative linear regression equation and correlation coefficient of the calibration graph for quantification of trans-resveratrol, as well as the results of replicate analysis of real samples and of a recovery study, carried out to evaluate precision and accuracy, respectively. The method is successfully applied to study the stability of trans-resveratrol in methanol solution under various storage conditions and to analyse commercial preparations of trans-resveratrol containing dietary supplements.