Study of multi-directional derivation of cord blood mononuclear cells and observation of killing activity to MGC-803 gastric cancer cell strain in vitro

Objective: To study multi-directional derivation of cord blood mononuclear cells to CD 3 AK, LAK and CIK cells as well as changes of killing activity to gastric cancer cell strain in vitro . Methods: CD 3 mAb and IL-2 were used to induce CD 3 AK cells, and IL-2 was used to induce LAK cells; IFN-γ was used in the beginning, then IL-1, CD 3 mAb and IL-2 were used to induce CIK cells after 24 h for observing amplification and analyzing their relationship. The phenotypes of the cultured CIK cells were analyzed by flow cytometry. Subsequently, by using MGC-803 gastric cancer cell strain as target cells, the killing activity of CD 3 AK, LAK and CIK cells was evaluated by using MTT method. Results: The amplification activity of CD 3 AK and CIK cells was all far higher than LAK cells ( P <0.05). The amplification activity had no obvious difference between CIK cells and CD 3 AK cells at prophase, but that was far higher in CIK cells than CD 3 AK cells at about 20 th day ( P <0.05). The flow cytometry revealed that the amount of CD 3 + CD 56 + cells, major effector cells after CIK cells being cultured was significantly increased ( P <0.05), moreover, the amount of CD 8 + cells was significantly increased as well ( P <0.05). The killing activities of CD 3 AK and CIK cells to the MGC-803 gastric cancer cell strain were all significantly higher than LAK cells, while the killing activity of CIK cells was stronger than CD 3 AK cells ( P <0.05). Conclusion: CIK cells have stronger amplification activity and killing activity, and can be taken as more effective killing cells applied to the tumor adoptive immunotherapy.