Detecting on effect of anti-tumor and the side reaction of sonosensitizer-loaded high polymer ultrasound contrast agents

Objective To observe the phototoxicities of hematoporphyrin-loaded PLGA ultrasound contrast agents(HP-PLGA)and to detect the effect of sonodynamic therapy on H22 tumor-bearing mice using HP-PLGA.Methods 35 Balb/c mice were divided into 5 groups,then different concentration of HP-PLGA microbubbles and hematoporphyrin solution were injected into each group of mice;after 15 minutes of sun irradiation,the phototoxicity of the eye,back skin,tail and liver of mice were observed,and the side reaction of liver was detected by HE stain.In addition,60 H22 tumor-bearing mice were divided into two groups(group A and group B).Then the two groups were again divided into five groups for different treatment evenly.The inhibition rate of tumor quality on the 15th day of group A and the life time of group B were detected,respectively.Results In mice injected into hematoporphyrin of 40 mg/ml and 20 mg/ml,the skin of the back reacted by forming a large area of eschar,swelling were observed in eyes and tails,and liver damage were seen in HE staining slice.Conclusions Hematoporphyrin-loaded PLGA ultrasound contrast agents used in sonodynamic therapy could inhibit growth of H22 tumor in mice and significantly reduce the phototoxicity side-effect of hematoporphyrin,which may provided a novel strategy for therapy on tumor.