A biosensor based on Thiobacillus thioparus for measuring thiosulfate and methanethiol

A biosensor based on Thiobacillus thioparus was developed for the determination of thiosulfate and methanethiol. Thiobacillus thiopants is a chemoautotrophic bacterium and it oxidizes sulfur compounds to sulfuric acid. The sensor consisted of an oxygen electrode and immobilized T. thioparus, When the sensor was used to determine thiosulfate, a linear relation between sensor output and concentration was obtained for the concentration range from 1 to 100 mu M in a batch system and from 1 to 10 mM in a flow injection system. Output of the sensor was stable for more than 1 month. For methanethiol, the response of the sensor was measured for the concentration range from 0.2 to 3 mM in a flow injection system.