Vera Schoft

The CRISPR lab at the Vienna Biocenter Core Facilities applied the CRISPR/Cas9 genome engineering system to A. thaliana as a basis for the design and production of customized transgenic plants and incorporated this into a service pipeline. Using our system, we observed heritable targeted mutagenesis frequencies of up to 85% in T1 plants. Furthermore, using three guides simultaneously, we managed to delete up to 12kb of genomic DNA in A. thaliana.