Molecular imaging is as an exciting area of multidisciplinary research that allows physiology and molecular processes to be visualized in living tissue. Instrumentation involved in molecular imaging includes magnetic resonance imaging (MRI), bioluminescence imaging (BLI), positron emission tomography (PET) and x-ray computer assisted tomography (CT). As part of the Center for Molecular Imaging, we are developing applications for imaging to allow for investigations of cancer growth and response to therapy including noninvasive detection of molecular signaling events in living tissue. Translation of developed algorithms into the clinical setting is ongoing.