Pilar Bustamante Madrid

PhD in 'Establishment of a cell differentiation system in organoids human colonics. Effect of vitamin D', under the direction of Prof. Alberto Muñoz and Dra. Asunción Fernández (Madrid, CSIC-UAM). I am specialised in organoids culture of stem cells derived from tumor and normal human colonic tissue, and performing treatments with drugs, cell differentiation factors or calcitriol. During these four years, I have completed my training with some courses (bioinformatics, good scientific practices, animal handling...) and organised a congress (II CIBERONC Youth Meeting). Among the laboratory techniques developed in this learning period, I highlight cell viability in 3D cultures, RNA analysis (RT-qPCR and RNAseq), protein analysis (western-blot and immunofluorescence), and ultrastructural phenotypic analysis (electron microscopy ).