Research Summary
Bioenergetics and cell biology of metabolically diverse, genetically-tractable bacteria. Biofilm biology in the context of human chronic infections and crop rhizospheres.

Electron-transfer reactions are fundamental to metabolism. Whether an organism is autotrophic or heterotrophic, free living or an obligate parasite, every cell must solve the energy-conservation problem to survive. At the cellular level, most of our knowledge of electron transfer comes from mechanistic studies of oxygenic photosynthesis and aerobic respiration in bacterial and eukaryotic systems. While we know in exquisite detail the structure and function of various membrane-bound proteins involved in electron-transfer processes (e.g., cytochrome c oxidase in mitochondria), we know far less about the electron-transfer agents of more ancient forms of metabolism that do not traffic in oxygen. As microbiologists interested in the origin and evolution of the biochemical functions that sustain modern life, our work has focused on probing the co-evolution of metabolism with Earth's near-surface environments. Understanding how modern microorganisms with archaic metabolisms function is a step towards this end. Moreover, because many biological microenvironments are anoxic, including those in most chronic infections, this path of inquiry leads inexorably to insights about cellular electron-transfer mechanisms that potentially have profound biomedical implications.

Because rocks provide the primary record of ancient events and processes, our laboratory initially explored microbe-mineral interactions. In particular, we investigated how bacteria catalyze mineral formation, transformation, and dissolution, focusing on how these processes relate to cellular energy conservation or membrane organization, and how they affect the geochemistry of their environment. For every pathway that we studied, we chose model organisms that we could genetically manipulate. Through a combination of classical genetic, biochemical, and molecular biological approaches, we identified the genes and gene products that controlled the processes of interest. As our work progressed, it became increasingly clear that our findings transcended microbe-mineral interactions. Accordingly, our focus has shifted to exploring basic physiological questions that are relevant to diverse biological systems. A geobiological perspective still imbues our approach, compelling us to perform our mechanistic studies under environmental conditions that bear fidelity to the complex contexts that motivate our work—be they the microenvironments of infected tissues or crop rhizospheres.