Bruno O. Villoutreix mostly deals with Drug discovery, Biochemistry, Bioinformatics, In silico and Virtual screening. The study incorporates disciplines such as The Internet, Information retrieval, Small molecule and Protein–protein interaction in addition to Drug discovery. Biochemistry is closely attributed to Factor V in his research.

The concepts of his Bioinformatics study are interwoven with issues in Web server, Adme tox and Lipocalin. His In silico research is multidisciplinary, incorporating perspectives in Chemical biology, Quantitative structure–activity relationship, Simplified molecular-input line-entry system, Cheminformatics and Computational biology. His study in the fields of Protein–ligand docking under the domain of Virtual screening overlaps with other disciplines such as Structure based.