Ribonucleic acid (RNA) molecules are fragile single-stranded transcripts, traditionally considered as the coding messenger needed to produce protein from the DNA. These RNA messengers (mRNA) have together with transfer RNA (tRNA) and ribosomal RNA (rRNA) historically been considered the essentials in RNA biology, driving the translation of RNA into protein. Today, it is known that only a minor part of all transcribed RNA is translated into protein and that several non-coding RNA species exist and have vital functions. These RNA species include long non-coding (lnc) RNA, microRNA (miRNA) and circular RNA.
Knowing the cellular origin, or subcellular localization, of a particular mRNA, miRNA, or lncRNA provides better understanding of the molecular mechanisms and related cellular context. Bioneer’s in situ hybridization services offer molecular histology services in a variety of disciplines with specific emphasis on RNA: in situ detection of mRNA, microRNA, and lncRNA using the best probe technologies on the market, including LNA and branched DNA technologies.