基本信息
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职业迁徙
个人简介
Courses Taught
Genetics 03-330
Molecular Biology 03-442
Virology 03-380
Cancer Biology 03-435
Research
Younis' lab is studying post-transcriptional regulation of gene expression, specifically intron splicing. The vast majority of human genes contain multiple introns that are removed by the spliceosome in order to connect the protein coding exons in frame. Introns are much larger than exons with vague sequence identifiers, making the task of intron splicing seems almost insurmountable, yet it is done with extreme precision in normal cells. When splicing mistakes occur, they often lead to disease. Splicing misregulation is also a hallmark of cancer cells.
The Younis lab uses a variety of technologies to characterize splicing and its regulation in normal and disease cells, including traditional techniques such as PCR and microarrays, as well as state of the art techniques such as Next Generation Sequencing of nascent pre-mRNA (RNA-seq) and High Throughput Screening (HTS). Using such methodologies, Younis discovered novel drugs that regulate splicing in cancer cells.
The Younis lab has been recently interested in a small set of introns, named minor introns, which are removed by a specialized spliceosome. Identification of all expressed minor introns in human cervical cancer cells led to the discovery that they function as stress-induced molecular switches that control the expression of the genes that harbor them. The mechanism for switching the splicing of minor introns ON or OFF relies on a highly unstable catalytic RNA, U6atac, which is a core component of the minor spliceosome required for the splicing reaction.
Genetics 03-330
Molecular Biology 03-442
Virology 03-380
Cancer Biology 03-435
Research
Younis' lab is studying post-transcriptional regulation of gene expression, specifically intron splicing. The vast majority of human genes contain multiple introns that are removed by the spliceosome in order to connect the protein coding exons in frame. Introns are much larger than exons with vague sequence identifiers, making the task of intron splicing seems almost insurmountable, yet it is done with extreme precision in normal cells. When splicing mistakes occur, they often lead to disease. Splicing misregulation is also a hallmark of cancer cells.
The Younis lab uses a variety of technologies to characterize splicing and its regulation in normal and disease cells, including traditional techniques such as PCR and microarrays, as well as state of the art techniques such as Next Generation Sequencing of nascent pre-mRNA (RNA-seq) and High Throughput Screening (HTS). Using such methodologies, Younis discovered novel drugs that regulate splicing in cancer cells.
The Younis lab has been recently interested in a small set of introns, named minor introns, which are removed by a specialized spliceosome. Identification of all expressed minor introns in human cervical cancer cells led to the discovery that they function as stress-induced molecular switches that control the expression of the genes that harbor them. The mechanism for switching the splicing of minor introns ON or OFF relies on a highly unstable catalytic RNA, U6atac, which is a core component of the minor spliceosome required for the splicing reaction.
研究兴趣
论文共 39 篇作者统计合作学者相似作者
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biorxiv(2024)
B. Memon, A. Elsayed, I. Bettahi, N. Suleiman,I. Younis, E. Wehedy, F. El-Ejeh,A. Abou-Samra,E. Abdelalim
引用0浏览0引用
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user-5dd52aee530c701191bf1b99(2020)
引用3浏览0引用
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作者统计
#Papers: 38
#Citation: 3399
H-Index: 21
G-Index: 32
Sociability: 5
Diversity: 3
Activity: 30
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