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First Molecular Evidence of Leishmania Infantum in Domestic Cats and Associated Risk Factors from the Black Sea Region of Türkiye

Didem Pekmezci, Alparslan Yildirim, Zeynep Nurselin Kot, Gamze Nur Konacoglu,Onder Duzlu,Gamze Yetismis, Sadullah Uslu, Seray Toz,Yusuf Ozbel,Abdullah Inci,Gokmen Zafer Pekmezci

Acta parasitologica(2024)

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摘要
The objectives of the present study are to determine the molecular prevalence of Leishmania spp. in the owned domestic cats in the Black Sea Region of Türkiye and analyze the associated risk factors in FeL. Conjunctival swabs (CS), blood, demographic, and clinical data were collected from 150 owned cats brought to the Veterinary Teaching Hospital during 2020–2022. Leishmania kinetoplast DNA (kDNA) from CS was screened by TaqMan Real-Time PCR (qPCR) with the genus-specific primers and a probe. All qPCR positive products were also amplified and sequenced to identify Leishmania species by ITS1 primers. Molecular prevalence of L. infantum found as 12.6
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关键词
Feline Leishmaniosis,Leishmania Infantum,Conjunctival swap,qPCR,Risk Factors,Türkiye
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