Electrotherapy; a promising therapy to eradicate viruses from infected in vitro olive cv. Meshkat

Olive tree harbors several viruses affecting the yield and quality of fruit worldwide. Application of virus-free planting materials is one of the main strategies to counteract virus diseases. The elimination of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Cucumber mosaic virus (CMV) and Strawberry latent ringspot virus (SLRSV) by different regimes of electrotherapy followed by 1 mm shoot tip culture was investigated in a native Iranian olive cultivar Meshkat. The results showed that survival, regrowth and proliferation rates were dependent on the therapy regime and shoot tip type. Apical shoot tips showed more significant proliferation rate compared to axillary ones under 30 min treatment of electric current. In addition, apical shoot tips represented higher survival and regrowth rate under 30 min of electrotherapy, 54 and 46