Near-Infrared Fluorescent Probe for the Detection of Cysteine
APPLIED SPECTROSCOPY(2024)
摘要
Hemicyanine dyes are an ideal structure for building near-infrared fluorescent probes due to their excellent emission wavelength properties and biocompatibility in biological imaging field. Developing a near-infrared fluorescent probe capable of detecting cysteine (Cys) was the aim of this study. A novel developed fluorescent probe P showed high selectivity and sensitivity to Cys in the presence of various analytes. The detection limit of P was found to be 0.329 mu M. The MTT assay showed that the probe was essentially non-cytotoxic. Furthermore, the probe was successfully used as cysteine imaging in living cells and mice.
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关键词
Hemicyanine dye,near-infrared fluorescent probe,cysteine detection,cell imaging,mice imaging
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