Circadian clock genes REV-ERB α regulates the secretion of IL-1 β in deciduous tooth pulp stem cells by regulating autophagy in the process of physiological root resorption of deciduous teeth

Physiological root resorption is a common occurrence during the development of deciduous teeth in children. Previous research has shown that the regulation of the inflammatory microenvironment through autophagy in DDPSCs is a significant factor in this process. However, it remains unclear why there are variations in the autophagic status of DDPSCs at different stages of physiological root resorption. To address this gap in knowledge, this study examines the relationship between the circadian clock of DDPSCs, the autophagic status, and the periodicity of masticatory behavior. Samples were collected from deciduous teeth at various stages of physiological root resorption, and DDPSCs were isolated and cultured for analysis. The results indicate that the circadian rhythm of important autophagy genes, such as Beclin-1 and LC3, and the clock gene REV-ERB alpha in DDPSCs, disappears under mechanical stress. Additionally, the study found that REV-ERB alpha can regulate Beclin-1 and LC3. Evidence suggests that mechanical stress is a trigger for the regulation of autophagy via REV-ERB alpha. Overall, this study highlights the importance of mechanical stress in regulating autophagy of DDPSCs via REVERB alpha, which affects the formation of the inflammatory microenvironment and plays a critical role in physiological root resorption in deciduous teeth.