Optimization and establishment of Agrobacterium-mediated transformation in alfalfa (Medicago sativa L.) using eGFP as a visual reporter

Efforts are still required for establishing an efficient and standard transformation procedure in alfalfa, a prominent forge crop worldwide, due to unstable repeatability of the published genetic transformation. The objectives of this study were to optimize and establish an effective genetic transformation system in alfalfa (Medicago sativa L.) using eGFP as a visual reporter, and test the feasibility of established procedure in other Medicago species. Comparisons of percent callus induction using three different explants of four alfalfa cultivars showed that hypocotyl explant had greater callus induction (70–98.6